WSTA 15th Gulf Water Conference

WSTA 15th Gulf Water Conference
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An alternative single plate-method for antibiotic residue screening in meat

Amina Tassist, Mahmoud Abouseoud, Nacéra Tadjine

https://doi.org/10.5004/ic202405

An alternative single plate-method for antibiotic residue screening in meat

Amina Tassist1, Mahmoud Abouseoud2, Nacéra Tadjine3

1Materials and Environment Laboratory, LME. Yahia Fares University, Medea, Algeria
2Faculty of Technology, Yahia Fares University, Medea, Algeria
3Faculty of Agronomy, Saad Dahleb University, Blida, Algeria

https://doi.org/10.5004/ic202405

ABSTRACT

The aim of our study is to develop an alternative, effective and economic multi-residue microbiological method for the screening of antibiotic residues in meat. Sixty-one samples were analyzed using the Premi®Test method and the (Four plate/STAR) method. The Premi®Test revealed 29.5% of positives against 70.5% of negatives. The (Four-plate/STAR) method gave 29.5% positives against 65.5% negatives and 5% doubtful samples. The one plate alternative method was developed on samples with Soy Agar and Mueller-Hinton using Micrococcus luteus, Bacillus stearothermophilus and Staphylococcus aureus. Under optimal conditions (Mueller-Hinton inoculated with Micrococcus luteus), the alternative method is competitive with the Premi®Test. It is promising for the screening of antibiotic residues, and does not detect false positives. It has a high sensitivity (Se = 0.85). It is also specific (Sp = 1). The positive (01) and negative (0.93) predictive values are also satisfactory.

Keywords: Four plate/STAR method; Screening methods; Premi®Test; One plate method; M luteus

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